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Unformatted text preview: CHEMISTRY Departmental Seminar Prof. Monika Sommerhalter California State University, East Bay Interception of damaged nucleotides by TM0159 from Thermotoga maritime Anna Desai, Khaldeyah Awwad, Monika Sommerhalter Chemical mutagens, UV light, ionizing radiation, and oxidative stress can damage DNA precursor molecules resulting in DNA mutagenesis and cancer. The protein TM0159 from Thermotoga maritima (a marine geothermal vent bacterium) is suggested to act as a control enzyme that specifically hydrolyses non-canonical (erroneous) nucleotides, for example XTP, ITP, dXTP, and dITP. This working hypothesis is based on the high structural similarity of TM0159 to other proteins that function as non-canonical nucleotide pyrophosphatases. We obtained a glycerol stock of recombinant E. coli cells with a plasmid harboring the TM0159 gene from the Joint Center of Structural Genomics (JCSG). TM0159 was equipped with an N- terminal polyhistidine tag, which greatly facilitated protein purification using affinity chromatography. terminal polyhistidine tag, which greatly facilitated protein purification using affinity chromatography....
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