041607_CE310setup - Worksheet Page:_ Date:_ Analyst:_...

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Worksheet Page:______ Date:_______________ Analyst:_____________ Setting up the ABI Prism 310 CE ___1. Check the log associated with the Power Macintosh on Instrument A to ensure that Norton Disk Doctor and Speed Disk maintenance is up to date. Note: Norton Disk Doctor is not conducted on Instrument B . If not, follow the directions in the Computer Maintenance Logbook to run these programs. ___2. If the ABI Prism 310 CE is off, make sure the computer is also off. Turn on the ABI Prism 310 CE and then turn on the computer. ___3. If the ABI Prism 310 CE is on, either turn on the computer or select RESTART from the SPECIAL menu. ___4. Check the instrument use log and the volume of polymer remaining in the syringe to determine whether the polymer needs to be changed. Approximately 2 μ L of polymer per injection is required. ___5. Open the ABI Prism 310 Collection program by either double clicking the icon or by highlighting and selecting open from the File menu after opening the hard drive folder. ___6. Select Change Capillary from the Instrument menu to determine whether the capillary needs to be changed. Record the number of injections indicated in the window on the CE Set-Up Sheet. Click Cancel to close. ___7. Remove the POP4 polymer from the refrigerator and allow it to come to room temperature. If precipitate is present in the bottle when first removed from the refrigerator, it should go back into solution at room temperature by mixing. Add lot number and control date to appropriate place in the software. ___8. Home the Autosampler: Choose Manual Control from the Window menu Select Autosampler Home X, Y Axis from the Function pop-up menu and choose Execute . Select Autosampler Home Z Axis from the Function pop-up menu and choose Execute . ___9. Select Syringe Home from the Function pop-up menu and choose Execute . ___10. Remove and clean the syringe: Open the instrument doors Move the syringe drive toggle to the left Unscrew the syringe from the pump block by grasping the metal collar. Do not unscrew by grasping the glass barrel of the syringe. Clean the syringe thoroughly by filling the syringe several times with deionized water and discarding the water.
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Worksheet Page:______ Date:_______________ Analyst:_____________ Dry the outside of the syringe with a Kimwipe . Store the syringe with deionized water in the barrel. Do not remove the plunger unless absolutely necessary to clean the syringe. If this needs to be done, replace the plunger into the syringe by placing a drop of deionized water on the Teflon seal and carefully insert the plunger into the body of the syringe. ___11. Remove the capillary Open the door covering the hot plate, remove the tape and pull the capillary through the electrode thumbscrew.
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This note was uploaded on 09/08/2010 for the course JS 113 at San Jose State University .

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041607_CE310setup - Worksheet Page:_ Date:_ Analyst:_...

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