Riboflavin Photometry Report

Riboflavin Photometry Report - What limits the linearity of...

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Riboflavin Photometry Report Use the standard format outlined in the document on writing reports. Include in your “Results” section the following: A plot of the absorbance at 444 nm vs. concentration of riboflavin taken from both the HP and Genesys20 spectrophotometers on the same figure, including linear regression lines. A plot of the absorbance at 372 and 268 nm vs. concentration of riboflavin on the same figure, including linear regression lines. A Table of all results: Molar absorptivities and associated standard deviations; absorption limits for the HP 8452A and Genesys20 spectrophotometers. Address in your “Discussion” section the following questions:
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Unformatted text preview: What limits the linearity of a Beers Law plot? In comparing the 444 nm data obtained from the HP 8452A and the Genesys20 spectrophotometers, which instrument is more precise? Which instrument is more accurate? In addition, answer the following questions: 1. A solution of riboflavin has an absorbance of 0.438 AU at 444 nm. What is the concentration of the riboflavin solution? 2. A solution of riboflavin has an absorbance of 3.48 AU at 444 nm. After diluting 1.000 mL of this solution to 10.00 mL, the absorbance of the diluted solution was measured to be 0.576 AU. What is the riboflavin concentration of the original (undiluted) solution?...
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