Problem 6 130C - c Why is it necessary for the 5’ capping of eukaryotic mRNA to occur well before the completion of synthesis d A eukaryotic cell

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Problem 6 (Due Monday, Apr. 5) a) Why can’t eukaryotic transcription be regulated by attenuation? b) A 15-nucleotide long strand of RNA has been synthesized that is complementary to segment 1 of trp L mRNA (this is the portion of the trp operon that codes for the leader peptide). What is the effect of the presence of this strand on the in vitro expression of the trp operon? Would the effect on expression be altered by the use of an operon in which a mutation in segment 2 of the trp L gene destabilizes the 2-3 stem/loop structure?
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: c) Why is it necessary for the 5’ capping of eukaryotic mRNA to occur well before the completion of synthesis? d) A eukaryotic cell carrying out transcription and mRNA processing is incubated with 32 P-labeled ATP as well as all other components necessary for the process. Specify the location of the label in the mature RNA molecules when the exogenously added ATP is labeled in the i) α , ii) β or iii) γ positions....
View Full Document

This note was uploaded on 09/08/2010 for the course CHEM 130C at San Jose State University .

Ask a homework question - tutors are online