dna_intro_part_3a_020707

dna_intro_part_3a_020707 - Slot blot hybridization Like in...

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Slot blot hybridization Like in yield gels, known amounts of DNA (human) are included DNA hybridization of D17Z1 will occur only if the sample contains human DNA Detection of the hybridized fragments is done using an enzyme linked assay- yielding light or color
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Real Time PCR
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Quantitative PCR- QPCR http://pathmicro.med.sc.edu/RTPCR/rt-pcr.ppt Real-time QPCR has several advantages over the other methods in that it is extremely accurate and sensitive over a broad dynamic range, and it occurs in a closed-tube system, reducing the potential for carryover contamination. Using this technique, a forensic biologist can monitor and quantify the accumulation of PCR products during log phase amplification. (Heid et al., 1996). Several RT PCR human specific assays are now available that target autosomal, Alu repeats, Y chromosome and mtDNA (Andréasson et al. 2002, Nicklas et al. 2003, Green et al. 2005, Andréasson et al. 2006, Horsman et al. 2006).
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This note was uploaded on 09/08/2010 for the course JS 113 at San Jose State.

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dna_intro_part_3a_020707 - Slot blot hybridization Like in...

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