131A_B LAB TALK.2

131A_B LAB TALK.2 - PCR 1. Denaturation (94 C) Top strand...

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Unformatted text preview: PCR 1. Denaturation (94 C) Top strand 5-acatcactgg agaaagtctt atg aaactcg cc gaacga actggtt taa tcttgccgct cccctgcatt-3 3-tgtagtgacc tctttcagaa tac tttgagc gg cttgct tgaccaa att agaacggcga ggggacgtaa-5 Bottom strand 2. Annealing (At least 5 C below the T M ) Top strand 5-acatcactgg agaaagtctt atg aaactcg cc gaacga actggtt taa tcttgccgct cccctgcatt-3 3- TGACCAA ATT CTTAAG GCGA GGGGAC-5 5- CTGG AGAA GGATCC ATG AAACTCG CC-3 3-tgtagtgacc tctttcagaa tactttgagc gg cttgct tgaccaa att agaacggcga ggggacgtaa-5 Bottom strand 3. Elongation (72 C optimal temperature for Taq polymerase) Top strand 5- acatcactgg agaaagtctt atg aaactcg cc gaacga actggtt taa tcttgccgct cccctgcatt -3 A tgtagtgacc tctttcagaa tac tttgagc gg cttgtc TGACCAA ATT CTTAAG GCGA GGGGAC-5 5- CTGG AGAA GGATCC ATG AAACTCG CC gaacga actggtt taa tcttgccgct cccctgcatt A-3 3- tgtagtgacc tctttcagaa tactttgagc gg cttgct tgaccaa att agaacggcga ggggacgtaa -5 Bottom strand 2 nd Round PCR 1. Denaturation 5end of gene: Top strand 5- acatcactgg agaaagtctt atg aaactcg cc gaacga actggtt taa tcttgccgct cccctgcatt-3 3- A tgtagtgacc tctttcagaa tac tttgagc gg cttgtc TGACCAA ATT CTTAAG GCGA GGGGAC-5 5- CTGG AGAA GGATCC ATG AAACTCG CC gaacga actggtt taa tcttgccgct cccctgcatt A-3 3- tgtagtgacc tctttcagaa tactttgagc gg cttgct tgaccaa att agaacggcga ggggacgtaa -5 Bottom strand 2. Annealing Top strand 5- acatcactgg agaaagtctt...
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131A_B LAB TALK.2 - PCR 1. Denaturation (94 C) Top strand...

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