profiler_plus_run_directions_validation

profiler_plus_run_directions_validation - User Bulletin...

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User Bulletin SUBJECT: AmpF l STR ® Profiler Plus™ ID PCR Amplification Kit Overview This User Bulletin describes the AmpF l STR Profiler Plus ID PCR Amplification Kit and experiments performed for its evaluation. In This User Bulletin This User Bulletin contains the following topics: Topic See Page Product Overview 3 Safety 5 About This Kit 7 How to Run Your Samples 11 Experiments Performed Using the AmpF l STR Profiler Plus ID PCR Amplification Kit 14 Developmental Validation 15 Species Specificity, Sensitivity, Stability, and Mixture Studies 18 Data Interpretation 26 Population Data 27 References 28
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Page 2 of 32 AmpF l STR Profiler Plus ID PCR Amplification Kit About This User Bulletin The AmpF l STR Profiler Plus ID PCR Amplification Kit User Bulletin provides supporting documentation for the AmpF l STR Profiler Plus ID PCR Amplification Kit. This user bulletin contains sections describing the following: Background information on the AmpF l STR Profiler Plus ID kit Experiments and Results The AmpF l STR Profiler Plus ID PCR Amplification Kit and the AmpF l STR ® Profiler Plus™ PCR Amplification Kit use the same protocols. This User Bulletin does not contain the general protocols necessary for amplification or fragment analysis on the ABI PRISM ® instruments. The AmpF l STR Profiler Plus PCR Amplification Kit User’s Manual (P/N 4303501) should be referred to for use with the AmpF l STR Profiler Plus ID kit. Chapters in the AmpF l STR Profiler Plus PCR Amplification Kit User’s Manual that are especially useful for the Profiler Plus ID kit are as follows: AmpF l STR Profiler Plus Kit Amplification (Chapter 5) ABI PRISM ® 377 DNA Sequencer Protocols (Chapter 7) ABI PRISM® 310 Genetic Analyzer Protocol (Chapter 8) Results and Interpretation (Chapter 9) Automated Genotyping (Chapter 10) Population Genetics (Chapter 13)
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AmpF l STR Profiler Plus ID PCR Amplification Kit Page 3 of 32 Product Overview Purpose The AmpF l STR Profiler Plus PCR Amplification Kit has proven to be extremely useful in applications for human identification. The AmpF l STR Profiler Plus PCR Amplification Kit co-amplifies the following nine short tandem repeat loci: D3S1358, D5S818, D7S820, D8S1179, D13S317, D18S51, D21S11, FGA, and vWA. Previously, Budowle (Budowle et.al ., 2000) reported an excess of homozygosity at the D8S1179 locus in a population of Chamorro and Filipinos from Guam. A single G-to-A transition (point mutation) was observed in all null alleles at the D8S1179 reverse primer-binding site. An additional D8S1179 reverse primer with a single nucleotide difference specific for the transition was included in the primer set of the newly developed AmpF l STR ® Identifiler™ PCR Amplification Kit to address this mutation. The new AmpF l STR Profiler Plus ID kit contains the same primers and uses the same reaction conditions and thermal cycling parameters as the AmpF l STR Profiler Plus kit with the addition of this second D8S1179 reverse primer. The addition of the primer
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This note was uploaded on 09/08/2010 for the course JS 113 at San Jose State University .

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profiler_plus_run_directions_validation - User Bulletin...

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