CHEMISTRY Departmental Seminar Ms. Nima Parmar San José State University Adviser: Dr. Joseph Pesek Characterization of Hydride-based Silica and Diamond Hydride Stationary Phases Aqueous normal phase chromatography has characteristics of Normal phase(NP) and Reverse phase(RP) chromatography. It can retain polar as well as non-polar compounds by carefully selecting the concentration ratio of organic solvent to water in the mobile phase. The separation of compounds takes place by interaction of the solute molecules with the stationary phase and mobile phase. Silica hydride phase stationary phases are becoming common due to their high pH stability, excellent efficiency and very good mechanical strength. Silica hydride columns do contain a small percentage
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This note was uploaded on 09/08/2010 for the course CHEM 285 at San Jose State University .