CHEMISTRY Departmental Seminar M.S. Final Seminar Ms. Prema Gangadhar San José State University Adviser: Dr. Joseph Pesek Synthesis and Characterization of Endcapped Stationary Phase Using Aqueous Normal Phase Liquid Chromatography and Reverse Phase Chromatography The silanization/hydrosilation process was developed to overcome some of the drawbacks associated with the use of organosilane reagents for the synthesis of high-pressure liquid chromatography stationary phases. The first step is called silanization in which silica or other oxides react with triethoxysilane (TES) resulting in the formation of silica hydride surface. Endcapping the stationary phase using trimethylchlorosilane (TMCS) and hexamethyldisilizane (HMDS) eliminates the unreacted and accessible silanols on the silica hydride intermediate. This research work employs the modification of bare silica to silica hydride via
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