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Unformatted text preview: -Dye blobs are artifacts that result from the fluorescent dyes coming off of their respective primers and floating through the capillary by itself.-Dye artifacts can be filtered out after PCR by using filtration columns.-Air bubbles, urea crystals and voltage spikes can also result in false peaks.-These peaks are usually not reproducible, so re-injection of the sample should solve the problem.-When interpreting the results to determine genotypes, the analyst should rely on validation studies, his/her own experience and literature results to understand anomalies on electropherograms.-When comparing two or more samples, three possibilities exist: a match, exclusion or inconclusive.-For matches, three other possibilities exist: the suspect deposited the sample, the suspect did not deposit the sample (population genetics) and the suspect did not deposit the sample but laboratory error resulted in a false positive (lab mistakes)....
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This note was uploaded on 09/08/2010 for the course JS 113 at San Jose State University .