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Unformatted text preview: iii) Dansylation followed by hydrolysis produced N(CH 3 ) 2 SO 2 NHCHCO 2 H CH(CH 3 ) 2 iv) Although treatment of I with either Trypsin or Chymotrypsin alone produced no fragmentation, simultaneous incubation with both enzymes resulted in the release of free Arg and an octapeptide (II) v) CNBr treatment of II produced a dipeptide and a six residue fragment (III) containing Cys, Gly, Pro, Tyr and Val. vi) Treatment of III with 2-mercaptoethanol produced two tripeptides (P 1 and P 2 ). One round of Edman degradation of P 1 produced PTH-Val, while the same treatment of P 2 produced PTH-Cys. In addition, P 1 showed a UV absorbance maximum near 280 nm. Use the above data to determine the amino acid sequence of I....
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This note was uploaded on 09/08/2010 for the course CHEM 135 at San Jose State.