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Problem 1 - 157.6 in 500mL of deionized water and dilute to...

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Problem 1 (Due Wed., Feb. 17) N- tris (hydroxymethyl)aminomethane (acronym TRIS, structure shown below) is commonly used as a biochemical buffer. The pK a of the protonated form of TRIS (TRISH + ) is 8.08. A laboratory manual has the following recipe for the preparation of 1.00L of .0500M pH 7.80 TRIS buffer: Dissolve 2.08g of TRIS (MW 121.1) and 5.17g of TRIS hydrochloride (MW
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Unformatted text preview: 157.6) in 500mL of deionized water and dilute to 1.00L. a) Verify that this recipe is correct. b) Calculate the pH of the solution obtained by adding 3.0mL of 1.0M NaOH to 500.0mL of the above buffer. TRIS: (HOCH 2 ) 3 CNH 2...
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