dna_intro_part_2b_020707

dna_intro_part_2b_020707 - Different DNA Extraction Methods...

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Different DNA Extraction Methods The organic method generally yields the highest quantity and quality DNA. The main disadvantages are that it is tedious with lots of steps and utilizes corrosive chemicals. The Chelex method is used when the sample contains very few cells and the reduced number of handling steps is the primary advantage. The main disadvantage is that it is yields crude DNA that is not as pure FTA paper is used to collect reference samples. It can be stored at room temperature, requires minimal handling and no quantification is required.
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Differential Extraction Method For Sexual Assault Evidence Isolation of DNA from mixtures of cells in sexual assault evidence Based on differences in cell membranes Spermatozoa membranes have special cross links (sulphur- sulphur bonds) These membranes are quite resistant to opening. Vaginal epithelial cells do not contain these membranes and are more easily broken open Sperm and v cell mixture Lysis- open v cell extract
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