TransformAid Manual - Home Search Contacts Order...

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Home Contacts Order Catalog Support Search Alphabetical Index Numerical Index Restriction Endonucleases Modifying Enzymes PCR Kits Markers Nucleic Acids Media Transfection Reagents Reagents C E R T I F I C A T E TransformAid™ Bacterial Transformation Kit #K2710 Lot: ___ Exp.: ___ Quantity: for 20 transformations #K2711 Lot: ___ Exp.: ___ Quantity: for 40 transformations PRECAUTION Wear gloves to avoid contact with skin IMPORTANT! Store at -20°C. Repeated freeze-thawing diminishes the quality. COMPONENTS* OF THE KIT 1. C-Medium: 35ml (70ml) of ready-to-use solution. 2. T-Solution ( A): 2.5ml (5ml) (2 (4) x1.25ml) of ready-to-use solution. 3. T-Solution ( B): 2.5ml (5ml) (2 (4) x1.25ml) of ready-to-use solution. * Do not contain glucose. STORAGE The T-Solution (A) and T-Solution (B) should be stored at -20°C. The C-Medium should be stored at - 20°C (in case of long -term storage) or at 4°C. For convenience, store the working C-Medium at 4°C. PROTOCOL Preparation of Bacteria from Overnight Culture
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1. Inoculate 2ml of TransformAid™ C-Medium with bacteria from a frozen stock or a colony. Incubate the culture at 37°C overnight. 2. Pre-warm culture tubes containing the required amount of TransformAid™ C-Medium (1.5ml for each of 2 transformations) at 37°C. 3. Add 1/10 volume of the overnight culture to the pre-warmed C-Medium (0.15ml overnight culture for each 1.5ml C-Medium). Incubate the tubes in a shaker at 37°C for 20min. Note A maximum of 26 transformations can be performed from 2ml of overnight culture. The culture can be kept at 4°C for at least a week and used for preparation of fresh cultures. Preparation of Bacteria from Bacterial Culture Plate 1. Inoculate a LB plate with bacteria using streak plate method, and incubate the plate at 37°C overnight. 2. Pre-warm culture tubes containing the required amount of TransformAid™ C-Medium (1.5ml for each 2 transformations) at 37°C. 3. Move a small portion of bacterial culture (4 x 4mm size for each 1.5ml of C-Medium) from the overnight LB plate using inoculating loop in to the pre-warmed C-Medium. Suspend the culture by gently mixing and incubate the tubes in a shaker at 37°C for 2 hours. Note
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This note was uploaded on 09/08/2010 for the course CHEM 132L at San Jose State University .

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TransformAid Manual - Home Search Contacts Order...

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