c135m3wl - Name ID I! Chem 135 Midtermfi Springllillu . a]...

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Unformatted text preview: Name ID I! Chem 135 Midtermfi Springllillu . a] (9 points} Draw the structure of a glycerophospholipid that has an lflrfl fatty acid in the snl position. a 20:3 ms fatty acid in the on: position and a serine esterified to the phosphate group that resides in the suit position. (This is an example of a phosphatidyl sense.) ‘9 t:- MnAl/‘NJ or Eat-,ka the We an 5H3 Cr b} to points} Draw the products of the hydrolysis reaction that occurs when dipaltttitoyl phospbstidylcholine is treated with phosphoiipase C. (Palmitate is the conjugate base of a [61} fatty acid.) ancfl EBt 1 +- FLOW“ DH" 3 D Wf to one PHessHoeHoLir-IE 2. a] (Fpoiats‘j When the membrane proteins on the sort'eoes of mouse and human cells are respecti rely labeled with green and red fluorescent markers and then allowed to fuse, initial images of the hybrid cells show green fluorescence on one half of the cell membrane and red fluorescenoe on the other. Visualizati on of the shells after a din-minute incubation at ETC: showed that the green and red markers were uniformly dispersed over the entire cell suri’aoe+ whereas incubation for an identical time period at [5“: showed the same neuter distribution as in the initial image. Aooount For these ohsenrations. W effl-I-[E‘D'MLLW I5”: W 31nd” Amm .r gfiflJ-{IEIHM' ,mvttsttdtm. (fits, Chem 13s Midterm 3 spring Jfllll Page 1 hf: {3 points] Assuming that both of the fluorescent markers are photnlabile_ show the time course of fluorescence emitted from a small area of the hybrid membrane subsequent to a bleaching laser flash in a phntebleaeh recovery experiment carried out ‘1} at ST“: and ii} at I5“C. (Two sketches are required.) F;WM 3. Recall that a typical value for the membrane potential of a eukaryotic cell is —tit] mm and that the Gibbs free energy change for transport of a solute X from one side et‘a membrane to the other is given by the expression showh below. AG = RTlnflXIzflX II + :Fdiq’ a} {ti patios] Calculate the value of the maximum concentration gradient that can be obtained by ATP—driven active transport of an unchanged solute into a cell. astuming that transport is driven by the hydrolysis ofone mole of ATPI per mole of solute transported. The value of AG For ATP hydrolysis under cellula: conditions is —5I12. Him-oi. {T = 31’C+ R = 8.3 [45 UK" mol']. F = 964351?" mol") AT EQ'II flqkmspark W EDI-2 itjmq:l =5, 5'11 kiwi. -: in ( [Ii-J”; $ HM? his: not ,r at = Q“. (bluffflnflmx at? (Dimiffldfl 3* e m. Hfi‘lr {5.1-1 kiwi" Mina/gastealyymg-)(3tnej e a? (technetium.- I'l than £35 Hitltet'm 3 Spring mm Page 3- b} [15 Willi-Iii Calculate the same maximum value as in part [at fur the AT F—ttriven transport of a Ca"1 inn but of the eel]. Infinite wan-ii. Mien-um fiWth-hmgm hhfltmcflg memfihw. up rfltfihfl‘f’ m {main- fi-qt = RThe([r:.-.@1Dutf[ca_@:lmj + z Fh'tp Went-infill. Hilfi- ei? e L(La®lflurf[en@]3 =e :11- ' W“ ( “u salami“; :l-r 1 [WEI-IE Eflfi Ifltflbflffl ItitttnaflilJEI-atufij Hm : E I'L- (*3 E9 = “e- iurr’fe 1-3..” e} {3 paints} The typical values far the innit and extracellular concentrations of New and K+ inns are It] mM and 145 [nit-l {NH} and MD mM and 5 mM (RH. Calculate the efficiency of the Na+il<+ ATPase by evaluating the freetien of the energy available from ATP hydrolysis {see part {3}} that is used to maintain the Nl-l- and K+ gradients across the plateau membrane. {Recall that the stniehitirnetrjiI of pumping is 3 NM but and 2 K4- in per ATP hydrolyzed.) “£31 baa-HE : “Th(tflyjuurll [Hg-ll“) + Fall“; I (5.51 II-ISI Film}: "lb-me.) {twin-t5} m‘} e (eel-mes inimit' Me. new) 1 (henna: 5-1quln3)1M"= t‘L'I him—el- andng i? 3M,£[.1.1LTM,E")= 3.5.131: I HE: WAKE = RTE-n (Kim-1m," Enfl3,w) + Fifi-“1' = {ht-Sinai Tye-Mi )[Ehfilflj Hm. (ti-text's} t- LHLHESIQLJ‘ Ill-newer) __ 3 '5) -t H'- El ‘ (5-5“! :0 -' 5-143.”- fl'm-E -. Leekflm; ZMKW =9? Eur-ffi (LEI: had”) = 5.“. LI Etc-1." flu—1"? + Dining 1(3n.|+5.hbjkir Lei-1313' -=‘p- win”? WWW 50-14139; :IJLL wm m WM» = can. tas atom... a Spring can Page 4 4. [1.5 points} The conversion of paimitate to palmitoyl EDA. is highly endergonic tit-16“: 3L5 Hinton. and has to be driven by ATP hydrolysis. in principle. the Formation of the EDA derivative could be driven by the hydrolysis of ADP to ATP and P,- {dfim= -3l].5 ltJa'moI} or to AMP and PH MED“: 42.2 kamttI]. In order to metabolize palmitate efficiently. the synthesis of the Cost derivative has to be essentially in'eversihle. Specify the route of palmitate activation {the synthesis of the (Sufi derivative} carried nut by the cell. and shovv that this is the proper choice by calculating the equilibrium constants associated 1with the two potential activation routes. [fiGD'= ‘RTlli Hi. pyrophosphatase is present in all cells; fifim uf PFi hydra] ysis is '335Uffl1fl” 9 ATP PADP D EOE-5H P; g EGUTE m: RAfiE—h .JhL, HHAD’?‘ L 1' : Erik-jag. t.- l‘ b * 3‘ all I " II I: “ET (flnFuFMfl-¥tnn + 3 (3L5 " END-5‘] iii-ME - 5 . s l-D kiwi fllvflllfi' Thus" 1;). 'bfln} E1: -(t.t:n: tzffiljfiifl-‘El‘tiifywét' :llil-tca figfime :c. = {DI-BE! ID 15 D same): as? 44;, RAMs/Ls RA FF; aha-Ich 4—-—-u-} 1?; Sam. 3 l- o I J o‘ 1 5|; NET = (finial-pnumnm + flc'krhnni' + flc' FF; ,1- __ "I = I it E} whet-Tin (fiqtinbljhfi'iftfi.5iH5iF/2M£I:)[1EDK‘] lie-q: 1: :r E ‘ 1 mm 1:4 mm MM fiH-EJ {‘3‘ HHLF.MILLLDM- =P' Rome 5:! in. oil... mow-.01. lu- mmchflfl Ineaveaalece ems; sm‘ {Ha-re at. In: a. Fla-514- HEcta-rTIv'E'. “Mn-m, Home. hag] anTEE) m Men-E I1- Wn‘ E“ E l',_—_' n _‘) Cherr- Iss Midterm s Spring me Page 5 5. a] [5 points] The mechanism of plant phosphoglyoerate mutase is different from that of the mammalian enzyme. 3—phosphoglycerate [3 PG} binds to the plant enzyme active site where the phosphate is first transferred to an active site residue and then transferred back to the glycerate Lposition to form 2P6. Descn'be the fate ofthe “a label when mist—sac. is added m i} hepatoeytes or ii] plant cells Emwinfi 1“ “mun-5r exFlaifling 3m": “ramming- InbtoACTI-H'I-T‘I'AM q. f PLANT: i 0 Fe' ‘5 @Pu' [ED " which I fifiwkoa fl, '3 5 g /\ry\ 5" 0'“ Hut/fig "'° F. '3' ‘1' [131?]: in". I 3'. I} c HHHML’. @r- a PI: o ' .F E. e. I,“ {a tween. hen-1t. to e VJ} is {El “0/3696 oe- 1; o'H a an; UNLAEELED 1mm. Inn-WM 5 mgmMHF—Ln- W.+ Fur-ulna” Emlmflsmm (gymnamau bi (6 points} The mechanian used by mammalian phosphogiucomutsse and phosphoglyeeromutsse are essentially the same. Explain the reason that occasional dissociation of the glucose—l .firbisphosphate intennediate from the active site inactivates the former enzyme. TILL [E3 hhh lHITthLL‘f F'Hnsrfiatl‘fLA‘rED fl... arerHosFI-tprre INTERHEDIH‘TE {mflshqueifll IEMW- :- I fill E u a m I ' l- uHPHHSPHaflVLn-‘I‘ED (E) .i... inane-rive mm id. M mf. oi flit-I.- ~W~ 94ml c} {3 points} Braeilin. a compound found in aqueous extracts of sappan wood. has been used to treat diabetes in Korea. This substance stimulates the activities of PFK-E and pyruvate itinase. Stipulate the effects of hmzilin on the glycolytic and glueoneogenie pathways in hepatocytes. and explain how these effects can be therapeutic for a patient suffering from diabetes. PFKL FALE-F'i hfiT|VhTE§ {#ng Mud. IHHIEth Ffifmi flan-IL- STtMuLnTIh-Ic. :L‘fany'su m Ir-h-‘I ID ITLHI: ELULDHEDGEHES '5 - + cups-3W Ehtm I35 Mid-“tarma- Spa-Ins 101! _ Pigtai- t5. flaunt atudiaa haw. shown that tip: halnphilia organism Hummus macaw-Myriam dagradaa glunnaa via tht: Entnar-Dautlnraff pathway [ahnwn balm-r]. a variant If the glycalytit: pathway uaad by Inc-at organisms. UH DH D Bill'ng NADFH Hfl HE! (1 HD LL!- ]IU- 63 —a- I] J} OH OH CH DH 1110 HQ [[0 0 ATP ADP [-[E. El “D L Pp WEE! W36 OH I] 13H 13 a A a o G _... —:-. —+ Parr-1W Em tic-ml Pall-Ht} 0}] a} {3 paints} What is the yield of ATP per male. of glunnaa far this pathway? [Explain your aaawarJ CINE ETF'Ja. ram M W FYR” ' was ad. GLYLEEALDEHV'pE-S-FHD'SPHA'I'E. [par-51* a.” Twuh'TF'a m... =‘p T-Iiu. MET "ftE‘I—D «3 ATP pue- b} [rpatntfl What impfll‘tflfll raactinns {in a general aanaa} must fallaw the raantinns of this pathway in under fan-the organism ta Survive? ...
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c135m3wl - Name ID I! Chem 135 Midtermfi Springllillu . a]...

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