dna_intro_part_3b_020707

dna_intro_part_3b_020707 - Comparison of Methods used for...

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Comparison of Methods used for DNA Quantification Method Ease Cost Sensitivity Result UV Spectrophotometry +++++ + ++ Total DNA Yield Gel electrophoresis +++ + + Int vs deg DNA Slot Blot ++ ++ +++ Human DNA Yield Gel blot + ++ +++ Int. vs. deg human DNA Pico-green microtitre plate ++++ ++ ++++ Total DNA Alu Quant +++ +++ ++++ Human DNA Real time PCR assays +++ +++ +++++ Human DNA Real time PCR assays +++ +++ +++++ Int.vs. deg. Human DNA
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Summary 1 (from last week) Why study DNA  Law enforcement, evolution, agricultural, and human  applications-medical diagnostics DNA Biology and Genetics – DNA is contained in  cells  –the basic unit of life – Found in  nuclei, mitochondria  and chloroplasts – Organized in  chromosomes .  Located at positions called  loci  and come in  different forms or  alleles .   Homozygous  if the same,  heterozygous  if different – Alleles  segregate independently  and  assort randomly
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This note was uploaded on 09/08/2010 for the course JS 113 at San Jose State.

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dna_intro_part_3b_020707 - Comparison of Methods used for...

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