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Unformatted text preview: 2 to achieve specified final concentration 5. 5 uL of genomic DNA 6. 0.4 uL HotStart Taq polymerase 7. Enough nuclease free H 2 O to bring the total volume to 50 uL Mix and spin briefly (with a pulse). Place tube into hot thermocycler immediately. Run thermocycler using the Trp program....
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This note was uploaded on 09/08/2010 for the course CHEM 132L at San Jose State University .