PCR Procedure F09 - 2 to achieve specified final concentration 5 5 uL of genomic DNA 6 0.4 uL HotStart Taq polymerase 7 Enough nuclease free H 2 O

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
PCR Procedure Pipette the following into a 0.5 mL PCR tube or a microcentrifuge tube (as instructed) on ice using barrier tips for all solutions: 1. 5 uL HotStart PCR buffer 2. 1uL dNTP mix 3. 2.5 uL of forward and reverse primers (10 uM stock; you will have to make this from 100 uM) 4. Appropriate volume of 25mM MgCl
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 2 to achieve specified final concentration 5. 5 uL of genomic DNA 6. 0.4 uL HotStart Taq polymerase 7. Enough nuclease free H 2 O to bring the total volume to 50 uL Mix and spin briefly (with a pulse). Place tube into hot thermocycler immediately. Run thermocycler using the Trp program....
View Full Document

This note was uploaded on 09/08/2010 for the course CHEM 132L at San Jose State University .

Ask a homework question - tutors are online