Tyrosine preparation

Tyrosine preparation - Chem 131A Preparation of Tyrosine...

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Chem 131A: Preparation of Tyrosine      The method used for the isolation of various amino acids consists of the hydrolysis of a protein mixture and  subsequent separation of the desired amino acid from the hydrolysate that contains other amino acids and  perhaps other substances.  In order to separate a particular amino acid, it is first necessary that the amino acid  is present in appreciable quantities in the protein, and secondly, that it possess properties, which facilitate its  separation from the other amino acids.      Amino acids and proteins are least soluble at their isoelectric points.  This fact is utilized in the preparation  of tyrosine from casein.   Although the isoelectric point of tyrosine is similar to those of other amino acids, it  may be separated from the mixture since it is present in relatively larger amounts in the protein used and is  appreciably less soluble than other amino acids present.      The preparation of tyrosine involves enzymatic hydrolysis of casein to yield tyrosine.
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Tyrosine preparation - Chem 131A Preparation of Tyrosine...

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