ORGANIClab1 - Experiment No 1 Name Paul Gonzales Experiment Name Thin Layer Chromatography Date Course CH 2123 Purpose the purpose of this lab is

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Experiment No.: Experiment Name: Date: 1 Thin Layer Chromatography 06/11/10 Name: Course: Paul Gonzales CH 2123
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Purpose: the purpose of this lab is to use Thin-Layer Chromatography (TLC) to separate pigments from a green leaf sample. Physical Data: MW Mp Bp Density Solubility Hazards Pet. Ether: eth. <-73 C 20-75 C Ins. in H20 Flamm. Anhy. MgSO4 120.366 1124 C 2.66 Sol. In H20 Calculations: N/A Procedure: 1. Place several fresh spinach leaves in a mortar and pestle, and add 10 mL 2:1 mixture petroleum ether: ethanol (bp 30-60) Grind leaves. 2. With pipette, transfer liquid to separatory funnel and swirl with an equal volume of H20. Don’t shake! 3. Separate. Discard lower aqueous phase. Repeat twice. 4. Transfer petroleum ether layer to small Erlenmeyer flask and add anhydrous sodium sulfate (use tip of spatula). 5. Decant solution from sodium sulfate, should have a deep green color, if not put under hood 15 min. 6. Obtain 10-cm by 2 cm strip cilica gel TLC plate. Place spot of pigment solution on sheet about 1.5 cm from one end using capillary tube to apply spot no larger than 2 mm diameter. 7. Let spot dry. Repeat to spot until dark green. 8. Develop chromatogram using 70:30 petroleum ether and acetone
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This note was uploaded on 09/07/2010 for the course CHEM CH 2123 taught by Professor Heemoonpark during the Summer '10 term at Austin Community College.

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ORGANIClab1 - Experiment No 1 Name Paul Gonzales Experiment Name Thin Layer Chromatography Date Course CH 2123 Purpose the purpose of this lab is

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