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Final Fall 2009

# Final Fall 2009 - BIS 102 Fall 2009 K Hilt Ka = x2(y-x 1 of...

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1 of 3 BIS 102 Name _____________________________________________ Fall, 2009 Last First K. Hilt Final Exam Score (200): ___________ Equations: pH = pK a + log {[b]/[a]} K a = x 2 /(y-x) K b = x 2 / (y-x) pH = (pK a 1 + pK a 2 )/2 (K a )(K b ) = 1 x 10 -14 F = (q 1 q 2 ) / ε r 2 G = H - T S v o = {V max [S]}/ {K m + [S]} Amino acid pK a ’s: α -carboxyl group (2.1), α -amino group (9.6) side chains: D (3.9) E (4.2) H (6.0) C (8.3) Y (10.1) K (10.5) R (12.5) Oligopeptide pK a ’s: C-terminal carboxyl group (3.6), N-terminal amino group (7.4) 1. (10 pts.) Liquid N 2 is sometimes used to break open cells, in order to begin the purification of a protein. How does liquid N 2 manage to break open cells? Explain briefly. Then give an example of when you would not want to use liquid N 2 and explain why not. 2. (30 pts.) Describe how you would purify hemoglobin from red blood cells. Assume that you start with 500 g of intact red blood cells. You are going to use an affinity column in your procedure. However, it is not your only step in the purification. When you get to your affinity column, discuss how you will make the resin, run the column, and elute it.

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Final Fall 2009 - BIS 102 Fall 2009 K Hilt Ka = x2(y-x 1 of...

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