sample midterm 1 2009 key

sample midterm 1 2009 key - YOUR NAME Student ID MIDTERM 1...

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YOUR NAME__________________________ Student ID#_________________ 1 NPB 100 MIDTERM 1 SPRING, 2009 Your signature______ KEY ________________ Please check this box if you do not want your exam returned in the bins in the hallway… Question Score Total 1 40 2 10 3 9 4 6 5 12 6 18 7 5 TOTAL 100
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YOUR NAME__________________________ Student ID#_________________ 2 Question 1. (40 points total) Here is a list of techniques. For each experiment below fill in the blank with one of these techniques. (Each technique may be used more than once or not at all!) Golgi stain, Patch clamp, Lucifer Yellow, Freeze fracture, IV curve, Immunohistochemistry, Tetrodotoxin (TTX), Nernst equation, Jellyfish fluorescent proteins, site-directed mutagenesis, time constant equation, space constant equation, Nissl stain, 4-AP, graph of conductance versus time, TEA, Voltage clamp, graph of conductance versus voltage, GHK equation, hydropathy plot. a) To calculate resting membrane potential you would use _______ GHK Equation ________ b) To count the number of dendrites on a hippocampal neuron you would use____ Golgi stain __ or _________ Lucifer yellow __ or ______ jellyfish fluorescent proteins ________________ c) To figure out how many membrane spanning regions are in a neurotransmitter channel you would use_____ hydropathy plot ________________ d) To see if cell bodies in the cerebellum are arranged in layers you would use ___ Nissl stain ___ e) To see whether one quantum of transmitter is released every time one vesicle fuses you would use _______ 4-AP _______________ and _______ freeze fracture ___________ f) To measure the current flowing through a leak potassium channel you would use _________ patch clamp ____________ g) To determine the conductance through a leak potassium channel you would use ___________ IV curve __________ h) To determine whether a channel is voltage-gated, you would use _____ IV curve _____or ________ graph of conductance versus voltage ______ i) To block voltage gated sodium channels you would use _______ TTX ________________ j) To stop a neuron from firing an action potential you would use ________ TTX ______________
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