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Unformatted text preview: 2 mm diameter (per technique, p. 703). 5. Use beakers (with filter paper liners) for elution chambers--keep ethyl acetate/acetic acid (99.5:0.5) solvent level below sample spots on plate when inserted into beaker; cover with foil and let stand in hood until solvent front reaches within 0.5 cm of top of plate. Remove plate and lay on paper towel to dry & immediately mark solvent front with pencil 6. Visualize eluted compounds in two ways: d. View under UV (short-wavelength light)--note colors, whether fluorescent or quenching, and outline spots with pencil e. Place in beaker with I2 crystals; cover with foil and place for two minutes in oven at 110 C. Return to hood to remove plate--observe colors and locations of spots. f. Measure Rf values (from starting line to spot centers) 7. Compare results with table on p. 108-109....
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- Spring '09