lecture note 10 - Model Organisms 16. Gene Expression in...

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1 16. Gene Expression in Prokaryotes and Eukaryotes Structure and Replication of Viruses Bacterial Genome and Gene Transfer Prokaryotic Gene Regulation Eukaryotic Genome Eukaryotic Gene Regulation K. SATHASIVAN Model Organisms Virions Come in Various Shapes Relative Sizes of Microorganisms The Lytic and Lysogenic Cycles of Bacteriophage Figure 13.4 The Lytic Cycle: A Strategy for Viral Reproduction
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2 Plant Viruses Transmission: Vertical: Generation to generation Horizontal: Within same generation Viral infection of plants Classes of Animal Viruses, Grouped by Type of Nucleic Acid The Reproductive Cycle of the Influenza Virus The Reproductive Cycle of HIV Measles
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3 Smallpox HIV infection BACTERIAL GENOME & GENETICS Bacterial chromosome and plasmids Genetic changes in bacteria Gene organization and regulation Plasmids Extra chromosomal, small and circular DNA R- Plasmid Antibiotic resistance F-Plasmid Fertility pilus for conjugation C-Factors Genes for toxic proteins Gene Transfer in Bacteria Transformation: uptake of DNA from surroundings Transduction: Phage mediated DNA transfer Conjugation: Bacterial mating between F+ and F- strains Transposition: Genes moving from plasmid to chromosome or vice versa Gene Transfer by Transformation and Transduction
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4 Gene Transfer by Conjugation Transposable Elements and Transposons Two Ways to Regulate a Metabolic Pathway lacY DNA Regulatory gene lacI Binds Lac repressor Binds RNA polymerase Promoter Operator Sequences that control the expression of the operon lacZ lac operon Transcription lacA The E. coli lac operon. Fig. 16-2, p. 331 Lac repressor Transcription initiation site β -Galactosidase Permease Transacetylase termination site lacA DNA RNA lacI a. Lactose absent from medium Promoter Operator Transcription blocked When lactose is absent from the medium, the active Lac lac operon lacZ lacY Fig. 16-3a, p. 332 mRNA Lac repressor (active) RNA polymerase cannot bind to promoter repressor binds to the operator of the lac operon, blocking transcription. Transcription
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This note was uploaded on 09/12/2010 for the course BIO 311C taught by Professor Sathasivan during the Fall '10 term at Austin Community College.

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lecture note 10 - Model Organisms 16. Gene Expression in...

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