Final Exam 2009 - Final Exam BIO362 Use the Opscan sheet...

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Final Exam BIO362 May 13, 2009 Use the Opscan sheet for your answers to these multiple choice questions. Write your name and ID number on the Opscan sheet, and be sure to fill in the little circles with your name and ID number. Otherwise the computer will not read your answers. 1. PCR: a. uses cycles of denaturation and renaturation. b. uses a DNA polymerase. c. uses oligonucleotides. d. All of the above are correct. e. Some, but not all, of the above are correct. 2. Uracil is not found in DNA a. because DNA polymerase cannot use dUTP. b. because dUTPase is a very active enzyme. c. because uracil-DNA glycosylase is an active enzyme. d. None of the above is correct. e. More than one of the above is correct. 3. Thymidylate synthase: a. converts uracil to thymine. b. converts dTDP to dTTP. c. converts dUMP to dTMP. d. converts UMP to dUMP. e. is inhibited by methotrexate. 4. The sliding clamp is important for DNA replication because: a. it makes primase more processive. b. it allows the leading and lagging strands to be coupled. c. it relieves torsional stress that arises during unwinding. d. it stimulates the replicative DNA helicase. e. it makes DNA polymerase more processive. 5. Primase: a. cannot start new chains. b. is a type of RNA polymerase. c. never acts at origins of DNA replication. d. is used exclusively at origins of DNA replication. e. is a type of reverse transcriptase. 1
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6. Telomerase: a. is a DNA polymerase. b. uses an RNA primer. c. uses an RNA template. d. All of the above are true. e. More than one, but not all, of the above are true. 7. Gout: a. is due to a defect in purine biosynthesis. b. is due to a defect in pyrimidine biosynthesis. c. is due to a protein-rich diet. d. is treated by inhibiting aspartate transcarbamoylase. e. None of the above is correct. 8. Ribonucleotide reductase: a. is responsible for making ribonucleotides. b. is responsible for making deoxyribonucleotides. c. is involved in the conversion of dUMP to dTMP. d. is inhibited by CTP. e. None of the above is correct. 9. Growing Okazaki fragments with their intact RNA primer: a. have a 5′ monophosphate end. b. have a 3′ triphosphate end. c. have a 5′ triphosphate end. d. never have uracil in the RNA . e. None of the above is correct. 10. E. coli cells carrying a plasmid: a. always are resistant to ampicillin. b. never are resistant to ampicillin. c. need tetracycline in order to grow. d. are sensitive to restriction enzymes. e. More than one of the above is correct. 11. The melting temperature of DNA: a. depends on the G+C content of the DNA.
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