20-Biol Plant-M-truncatula-SSR

20-Biol Plant-M-truncatula-SSR - BIOLOGIA PLANTARUM 53 (2):...

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BIOLOGIA PLANTARUM 53 (2): 343-346, 2009 343 BRIEF COMMUNICATION Genetic diversity assessment in Greek Medicago truncatula genotypes using microsatellite markers P. AKRITIDIS*, P.V. MYLONA**, A.S. TSAFTARIS* , *** and A.N. POLIDOROS* ,1 Institute of Agrobiotechnology, CERTH, Thermi GR-57001, Greece* Agricultural Research Center of Northern Greece, NAGREF, Thermi GR-57001, Greece** Department of Genetics and Plant Breeding, AUTh, Thessaloniki GR-54006, Greece*** Abstract In this study we examined the genetic diversity and geographic scale of genotype distribution within the model legume species Medicago truncatula widely distributed in pasture and marginal agricultural lands in Greece and other Mediterranean countries. Thirty one Medicago truncatula and Medicago littorialis accessions were chosen on the basis of their geographical distributions and studied using 9 polymorphic simple sequence repeats (SSR) markers. The number of alleles per locus varied between 3 and 7. A total of 42 alleles were detected with a mean value of 4.66 alleles per locus. Geographic origin was not related with genotypic similarity among accessions. However, there were instances of close genetic relatedness between accessions from neighboring locations in a geographic compartment. In conclusion, the presented data revealed extensive M. truncatula genotype dispersal in Greece pointing to the significance of preserving local genetic resources in their natural environment. Additional key words : molecular markers, phylogenetic analysis, simple sequence repeats. ⎯⎯⎯⎯ Germplasm characterization is important for evaluation of genetic resources and utilization of valuable genotypes for breeding purposes. Medicago truncatula, a close relative of alfalfa, has gained much attention in plant research serving as a model species in legumes, like Arabidopsis in non-legume dicot plants. M. truncatula is also important as a forage legume crop especially in Australia. Genetic diversity within a species especially in locations where it is endemic and represent a center of origin or initial distribution must be evaluated and preserved (Mirali et al. 2007). Microsatellites or simple sequence repeats (SSRs) have become the preferred markers to examine genetic diversity in many studies (Dikshit et al. 2007, Joshi and Dhawan 2007) since they exhibit high levels of variability, ease and reliability of scoring, co-dominant inheritance and short lengths (Sunnock 2000). Polymorphic microsatellites have been isolated and characterized in M. truncatula (Baquerizo- Audiot et al. 2001; Choi et al. 2004, Mun et al. 2006). SSRs have been used to examine population polymorphisms revealing an unexpected large amount of intrapopulation polymorphism at specific locations in this self-fertilizing species (Bonnin et al. 2001). SSRs have also been used to evaluate genetic diversity in large germplasm collections maintained by the South Australian Research and Development Institute (SARDI) of the Australian Medicago Genetic Resources Center
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This note was uploaded on 09/13/2010 for the course DGPB 024e taught by Professor Alexiospolidoros during the Spring '10 term at Aristotle University of Thessaloniki.

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20-Biol Plant-M-truncatula-SSR - BIOLOGIA PLANTARUM 53 (2):...

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