19-EJB-GMO PAPER-ms - Electronic Journal of Biotechnology...

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Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.11 No.2, Issue of April 15, 2008 © 2008 by Pontificia Universidad Católica de Valparaíso -- Chile Received July 20, 2007 / Accepted November 13, 2007 This paper is available on line at http://www.ejbiotechnology.info/content/vol11/issue2/full/11/ DOI: 10.2225/vol11-issue2-fulltext-11 RESEARCH ARTICLE Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method Paschalis Akritidis Institute of Agrobiotechnology Centre for Research & Technology Hellas 6th Klm. Charilaou - Thermi Road P.O. BOX 60361 GR - 570 01 Thermi, Thessaloniki, Greece Tel: 30 2310 498272 Fax: 30 2310 498270 E-mail: paschalis@certh.gr Konstantinos Pasentsis Institute of Agrobiotechnology Centre for Research & Technology Hellas 6th Klm. Charilaou - Thermi Road P.O. BOX 60361 GR - 570 01 Thermi, Thessaloniki, Greece Tel: 30 2310 498274 Fax: 30 2310 498270 E-mail: kpasents@certh.gr Athanasios S. Tsaftaris Institute of Agrobiotechnology Centre for Research & Technology Hellas 6th Klm. Charilaou - Thermi Road P.O. BOX 60361 GR - 570 01 Thermi, Thessaloniki, Greece Tel: 30 2310 498271 Fax: 30 2310-498270 E-mail: tsaft@certh.gr Photini V. Mylona Agricultural Research Center of Northern Greece National Agricultural Research Foundation Georgikis Sholis Avenue 57001 Thermi, Greece Tel: 30 2310-471613 Fax: 30 2310-498270 E-mail: phmylona@nagref.gr Alexios N. Polidoros* Institute of Agrobiotechnology 6th Klm. Charilaou - Thermi Road P.O. BOX 60361 GR - 570 01 Thermi, Thessaloniki, Greece Tel: 30 2310 498273 Fax: 30 2310 498270 E-mail: alexios@certh.gr Urls: www.certh.gr / www.nagref.gr Financial support: This work was supported by a PENED grant (01 ΕΔ 569) awarded to A.N.P from the General Secretariat of Research and Technology (GSRT) of Greece. Keywords: event specific detection, genetically modified cotton, genome walking, GMO detection, Mon1445. Abbreviations: CaMV: cauliflower mosaic virus EU: European Union GMO: genetically modified organism NGO: non-governmental organization PCR: polymerase chain reaction
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Akritidis, P. et al. 2 Entering the second decade of commercialization of biotech crops, the global area cultivated with transgenic plants constantly expands and national legislations in many countries, particularly in the European Union, require identification and labeling of genetically modified material in food and feed. We describe here a procedure for characterizing transgenic material of unknown origin present in conventional seed lots using a genome walking strategy for isolation and characterization of the junction between the inserted transgene construct and the host plant genomic DNA. The procedure was applied to transgenic cotton
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19-EJB-GMO PAPER-ms - Electronic Journal of Biotechnology...

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