Chapter 1 - Basics of Replication

Chapter 1 - Basics of Replication - Francis Crick and James...

Info iconThis preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Francis Crick and James Watson Review of the Basics of Bacterial Chromosome Replication and Structure Polarity and Antiparallel Orientation of Strands in DNA 3 5 A B A B 3 5 3 5 DNA Replication My Heroes In 1953 Watson and Crick PREDICTED that DNA replication is semi- conservative , meaning that each strand can act as a template for the synthesis of its complementary strand by a process that takes advantage of the rules of base pairing: two new copies are made, each consisting of an old (template) strand and a new strand. In 1958, Matthew Messelson and Franklin Stahl confirmed experimentally that DNA replication is semiconservative. Polymerization of Deoxyribonucleoside Triphosphates (dNTPs) as dNMPs during DNA Synthesis The - diphosphate (pyrophosphate) is cleaved to provide the energy required for formation of a phosphoester bond by the phosphate of the dNTP with the 3 carbon at the end of a growing DNA strand. Fig. 1.6 HO HO Some corrections Protein Gene Function DnaA dnaA Initiator protein, primosome (priming complex) formation DnaB dnaB DNA helicase (strand separation; hexamer) DnaC dnaC Delivers DnaB to replication complex SSB ssb Binding to single-stranded DNA Primase dnaG RNA primer synthesis DNA Pol III 11 genes DNA replication (presented in next slide) DNA Pol I polA Primer removal, gap filling DNA ligase lig Sealing DNA nicks DNA gyrase DNA supercoiling (unwinding) subunit gyrA Nick closing subunit gyrB ATPase Topo I topA DNA supercoiling (DNA packaging) Pol III TABLE 1.1 Proteins involved in E. coli DNA replication a Full-length translation product of dnaX gene b Shorter product of dnaX gene produced by translational frameshifting (Chapter 2) Subunit Gene Function dnaE Polymerization (catalytic subunit). dnaQ 3 5 editing exonuclease. RNase H rnhA Removes RNA primers? Endonuclease that cleaves RNA in RNA/DNA duplexes. Primer removal in eukaryotes. holE Present in core. dnaN Sliding clamp. a dnaX Organizes complex; joins leading and lagging DNA PolIII complexes. b dnaX Clamp loading. holA Clamp loading. holB Clamp loading. holC Clamp loading. holD Clamp loading. TABLE 1.1 The 11 polypeptides of the E. coli DNA Pol III (holoenzyme) A. All DNA polymerases synthesize DNA strands from the 5-end to the 3-end....
View Full Document

This note was uploaded on 09/14/2010 for the course MMG 431 taught by Professor Bertrand during the Fall '08 term at Michigan State University.

Page1 / 30

Chapter 1 - Basics of Replication - Francis Crick and James...

This preview shows document pages 1 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online