Activity_1.1.5_ELISA_New.docx - Activity 1.1.5 ELISA INTRODUCTION Given Sue\u2019s diagnosis all of the patients seen in the past two days need to be

Activity_1.1.5_ELISA_New.docx - Activity 1.1.5 ELISA...

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Activity 1.1.5 ELISA INTRODUCTION Given Sue’s diagnosis, all of the patients seen in the past two days need to be called back in for immediate testing. School officials are concerned about a possible outbreak of bacterial meningitis on campus. To diagnose bacterial meningitis, it is necessary to obtain a sample of cerebral spinal fluid using a spinal tap. Since this procedure is extremely invasive and painful, only those patients whom doctors feel are at greatest risk for the disease will be tested. Use the spinal fluid samples to identify those who are infected with meningitis and to trace how this disease may have spread among the students on campus. Devise a plan to halt the spread of the disease before it is too late! In Human Body Systems, you investigated the workings of the immune system and learned how antibodies , specific proteins produced in response to invading antigens, circulate to keep us healthy. Antibodies seek out and attach themselves to invaders, flagging them for destruction by the immune system. These antigens are molecules foreign to the body and can include bacteria, viruses, and fungi. Because antibodies are extremely specific to the antigens they attack, these proteins can be used in the laboratory to help identify disease agents. One test, the Enzyme-Linked Immunosorbant Assay (ELISA) , combines targeted tagging with antibodies and an enzyme reaction that produces a visible color change, to test for the presence of disease antigens or antibodies produced in response to that antigen. The ELISA can even detect disease agents in body fluids before the body has a chance to mount an immune response and produce antibodies. An ELISA can provide qualitative results , indicating whether a patient is positive or negative for the presence of the antigen or antibody , or an ELISA can provide quantitative results , determining how much of the detected substance is present. In this lab, you will use ELISA to test simulated cerebral spinal fluid (CSF) samples taken from patients at Sue’s school for the presence of bacterial meningitis. This rapid test can be completed in less than one hour and can detect antigens of the Neisseria meningitidis bacteria. Your job is to determine which college students are infected with this deadly bacterium and to propose a strategy for halting further spread. For those who are infected, use quantitative data to propose a chain of infection. Use data from the ELISA test and from the patient histories to trace how this disease may have spread from person to person.
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  • Fall '16
  • Joseph Begeny

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