Lecture_10_2-23-10-PDF_40838

Lecture_10_2-23-10-PDF_40838 - Review Which complex is...

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Which complex is unable to generate sufficient free energy to power ATP synthesis? A) Complex I B) Complex II C) Complex III D) Complex IV E) All the complexes are able to generate sufficient free energy. Review
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Complex I (46 subunits) NADH + CoQ (oxidized) NAD + + CoQ (reduced) Complex III (11 subunits) CoQ (reduced) + 2 cytochrome C (oxidized) CoQ (oxidized) + 2 cytochrome C (reduced) Complex IV (13subunits) 2 cytochrome C (reduced) + ½ O 2 2 cytochrome C (oxidized) + H 2 O Complex II (5 subunits) FADH 2 + CoQ (oxidized) FAD + CoQ (reduced) D E = 0.085 V, D G o’ = -16.4 kJ/mol Not sufficient to synthesize ATP. Only to inject electrons from FADH 2 cf. ADP + Pi ATP D G o’ = 30.5 kJ/mol Review
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Complex I (46 subunits) NADH + CoQ (oxidized) NAD + + CoQ (reduced) Complex III (11 subunits) CoQ (reduced) + 2 cytochrome C (oxidized) CoQ (oxidized) + 2 cytochrome C (reduced) Complex IV (13subunits) 2 cytochrome C (reduced) + ½ O 2 2 cytochrome C (oxidized) + H 2 O Complex II (5 subunits) FADH 2 + CoQ (oxidized) FAD + CoQ (reduced) D E = 0.085 V, D G o’ = -16.4 kJ/mol Not sufficient to synthesize ATP. Only to inject electrons from FADH 2 Review
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Subunit composition of complex I (46 subunits in bovine) SDS-PAGE Supplemental
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p558, BOX 17-1 415 521 550 ? Perspectives in Biochemistry p610, BOX 18-1
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Absorbance spectrum of Cytochrome C (purified) 415 521 550 p610, BOX 18-1
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Cytochrome c (purified) Membrane (crude) Identification of protein species in the crude sample p610, BOX 18-1
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Spectrophotometer p610, BOX 18-1
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Biochemical fractionation of mitochondria into separate components:1 Supplemental
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Biochemical fractionation of mitochondria into separate components: 2 Supplemental
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Biochemical fractionation of mitochondria into separate components: 3 Supplemental
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Biochemical fractionation of mitochondria into separate components: 4 Supplemental
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Biochemical fractionation of purified mitochondria into separate components: Final samples 1 2 3 4 Measure absorbance spectrum Supplemental
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Complex I (46 subunits) NADH + CoQ (oxidized) NAD + + CoQ (reduced) Complex III (11 subunits) CoQ (reduced) + 2 cytochrome C (oxidized) CoQ (oxidized) + 2 cytochrome C (reduced) Complex IV (13subunits) 2 cytochrome C (reduced) + ½ O 2 2 cytochrome C (oxidized) + H 2 O Complex II (5 subunits) FADH 2 + CoQ (oxidized) FAD + CoQ (reduced) D E = 0.085 V, D G o’ = -16.4 kJ/mol Not sufficient to synthesize ATP. Only to inject electrons from FADH 2
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3. Oxidative Phosphorylation A. The Chemiosmotic Theory B. ATP Synthase C. The P/O ratio D. Uncoupling oxidative phosphorylation Lecture 10
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Oxidative Phosphorylation Electron transport is responsible for the production of ATP 1. Chemical Coupling Hypothesis : Is electron transport system (ETS) directly coupled (through an isolatable intermediate ) to ATP production? or 2. Chemiosmotic Coupling Hypothesis : Is electron transport system (ETS) indirectly coupled to ATP production?
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Lecture_10_2-23-10-PDF_40838 - Review Which complex is...

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