Lab microscopy cells

Lab microscopy cells - LABORATORY MICROSCOPY CHEEK CELLS Purpose Objectives • To observe high magnification images of subcellular structures

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LABORATORY - MICROSCOPY & CHEEK CELLS Purpose & Objectives To observe high magnification images of subcellular structures using microslides. To identify parts of a microscope. To describe the purpose of microscopes. To describe the relationship between magnification and resolution. To prepare slides of living cells for observation under the microscope. To describe the size of cells. Background Microscopes are designed to make objects visible that are too difficult or too small to with the human eye. Microscopes are used by all types of scientists. Different kinds of microscopes are designed to visualize at different magnifications and different types of specimens. We will be using the compound microscope in this lab. Examine the diagram of the structure of a compound microscope at the end of this laboratory. The term compound refers to the two magnifying lenses present. One lens is in the eye-piece(s) (binocular if there are two eyepieces, monocular if there is one eyepiece) and the other lens is called the objective lens. The magnification of a compound scope is achieved through the interplay of the two lenses. The total magnification of any specimen being viewed is equal to the power of the ocular lens multiplied by the power of the objective lens. For example, if the ocular lens magnifies at 10X (where X implies 10 times magnified ) and the objective lens magnifies at 63X, the total magnification is 10 multiplied by 63 or 630X. Compound microscopes use an illumination light (the type of light can vary: regular light, UV light, fluorescent light) to aid in visualizing the specimen. When used and maintained correctly, microscopes can allow you to clearly see intricate the structures of biological specimens. Always carry the microscope in an upright position using two hands. Grasp the arm with one hand and support the base with the other. Microscope lenses must only be cleaned with lenses paper to prevent scratch marks. Compound microscopes can usually magnify an object up to about 400X although some objective lenses can magnify objects at 1000X. The higher the magnification, the more expensive the lens. Compound microscopes can be used to examine prepared slides of specimens that already have a cover slip or to
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This note was uploaded on 09/16/2010 for the course BIO 1A taught by Professor Ahuja during the Summer '10 term at Laney College.

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Lab microscopy cells - LABORATORY MICROSCOPY CHEEK CELLS Purpose Objectives • To observe high magnification images of subcellular structures

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