MT SP06 montal (2)

MT SP06 montal (2) - BIBC 100 Fall 2006 Final/Montal Name:...

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BIBC 100 Name: _________________________________________ Fall 2006 Final/Montal ID: ____________________________________________ - 1 - 1. Identify the protein that is depicted on the screen.(6) SH3 a. Name a Tyrosine Kinase protein that contains this motif? (2) Src b. List the other domains within this Tyrosine Kinase protein, what are the structures of each motif?(8) SH2— α/β motif Small kinase domain, Large kinase domain— α -helical c. What are the functions for each domain of this Tyrosine Kinase protein?(6) SH2: binds to phosphorylated tyrosines SH3: binds to proline rich region, polyproline type II helix Kinase domain: phosphorylates Tyrosine residues d. What is the function of this Tyrosine Kinase protein?(2) To phosphorylate proteins, signal transduction e. How does this Tyrosine Kinase protein regulate its own function?(6) Own SH2 domain bound to own phosphorylated C-terminal tyrosine is a inactive conformation. To become active, conformational change to active conformation by dephosphorylation of this tyrosine to release SH2 domain.
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BIBC 100 Name: _________________________________________ Fall 2006 Final/Montal ID: ____________________________________________ - 2 - 2. Chymotrypsin is incubated with the following substrate: N terminus - M-Q-D-H-N-R-R-S-C-Y-Q-A-V -C terminus . Write out the sequence of the cleavage products. (4) N terminus - M-Q-D-H-N-R-R-S-C-Y -C terminus N terminus -Q-A-V -C terminus a. How many peptide bonds are in each cleavage product?(4) 9 peptide bonds and 2 peptide bonds b. Which amino acids determine the specificity to cleave the scissile bond of this peptide?(4) Tyrosine c. How does Chymotrypsin differ from Cysteine proteases?(4) Serine in the catalytic triad of Chymotrypsin vs. Cysteine in the Cysteine proteases d. If you added a PH domain onto Chymotrypsin, how would it modify the function of the protein? (2) Chymotrypsin is now localized to the membrane, only cleaves substrates that are present on the plasma membrane
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BIBC 100 Name: _________________________________________ Fall 2006 Final/Montal ID: ____________________________________________ - 3 - 3. For the following classes of proteins list a specific protein studied in the papers assigned to this class and what technique was used to determine its structure. a. Ligand gated ion channel(4) Acetylcholine receptor b. Voltage gated ion channel (4) KvAP c. Channel that translocates polypeptides (4) Sec61 or SecY d. Where, with respect to the membrane, do polypeptides exit the channel listed in part (c)?(4) To ER lumen if soluble protein and into the membrane if a membrane protein 4. In order for serine proteases to catalyze the cleavage of a peptide bond, specific structural requirements need to be present in the enzyme. What is the name for the group of three critical amino acids present in the active site of a serine protease?(2) Catalytic Triad a. Name the three amino acids and state their function in the catalysis. (6) Ser: form covalent bond w/ substrate, His: accepts H
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This note was uploaded on 09/18/2010 for the course BIBC 100 taught by Professor Nehring during the Spring '07 term at UCSD.

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MT SP06 montal (2) - BIBC 100 Fall 2006 Final/Montal Name:...

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