Practice Test 5

Practice Test 5 - Biol-1107 Armstrong Test#5-9 AM 1 What is...

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Biol-1107 Armstrong Test #5-9 AM Fall, 2005 1) What is the enzymatic function of restriction enzymes? A) to join nucleotides during replication B) to cleave nucleic acids at specific sites C) to add new nucleotides to the growing strand of DNA D) to join nucleotides during transcription E) to repair breaks in sugar-phosphate backbones 2) Which of the following is NOT true of plasmids A) it is easy to get them into and out of bacteria. B) they are used to carry fragments of DNA researchers are studying. C) they are made of RNA D) they are much smaller than the main bacterial chromosome. E) All of the above are true of plasmids 3) Which of the following would part of a pallindromic DNA sequence? A) 5'GGGTTT3' B) 5'GGCCGG3' C) 5'GAATTC3' D) 5'GCCCCG3' E) 5'GGGGGG3' 4) Some people are violently opposed to the production and use of genetically modified (GM) foods because A) the American Medical Association requires labeling of all GM foods. B) the foods increase productivity and thus fewer people will starve. C) it is important to stop the use of GM crops before use becomes widespread. D) it is impossible to predict all of the effects changing the genetic make up of an organism might have. E) studies have shown that people often develop allergic reactions to the genetic changes that have been made to foods. 5) A eukaryotic gene has "sticky ends" produced by the restriction enzyme Eco RI. The gene is added to a mixture containing Eco RI and a plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for Eco RI located in the middle of the tetracycline resistance gene. The mixture is incubated for several hours, exposed to DNA ligase, and are then streaked onto a plate such that individual bacteria multiply to form colonies. Each colony contains thousands of bacteria that are identical to the bacteria that originally landed at that location. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics. Bacteria that contain the plasmid, but without the
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Practice Test 5 - Biol-1107 Armstrong Test#5-9 AM 1 What is...

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