21_pdfsam_apluspreptt1pkg - ph = 8-9 > subunits...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
ph = 8-9 > subunits DISSOCIATE due to repulsion between -COO - ‘s Icosahedral IONIC interactions most important Add NaCl > NH 3 + 4 - neutralized > subunits DISSOCIATE ++ > ionic bonding ++ > HYDROGEN bonds > NORMAL viral particle ++ > NO H + bonds between -COO - ‘s > repulsion > viruses SWELL & S DECREASES (i.e. sediment more slowly) > subunits do NOT dissociate as RNAs hold them together pH 7, w/ 1M NaCl > ionic interactions disrupted > DISSOCIATE STEP FIVE: Divided Genome TRV – short: capsid for BOTH long & short; long: polymerase for BOTH short alone > NOT infectious long alone > disease symptoms but NO viral particles are made BMV – RNA3&4 in the same capsid; RNA4 is a subgenome of RNA3 RNA3 > dicistronic, make subgenomic RNA4 > coat protein RNA4 > does NOT replicate through RF RNA1, 2, 3 added together > INFECTIOUS (i.e. do not need 4) cf. Density: 1 > 3+4 > 2 (in equilibrium centrifugation) AMV – Polymerase of AMV requires CAPSID to become functional
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/19/2010 for the course CSB CSB351 taught by Professor Ab during the Spring '09 term at University of Toronto- Toronto.

Ask a homework question - tutors are online