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34_pdfsam_apluspreptt1pkg - plant cells the plant shows...

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B. 1, 2, 3 C. 3 D. 3, 4, 5 E. 5 19. Which statement(s) is/are true about recombinant DNA technology? 1. Expression of the genes introduced by plasmid vectors is dependent upon host chromosome replication 2. Both microinjection and biolistics techniques utilize protoplasts 3. All the cells of transgenic plant express plasmid-introduced genes, as A. tumefaciens infecting plantlets introduce Ti-plasmids into all the plantlet cells 4. Binary vectors have antibiotic resistant genes and a origin of replication, and can grow both in E. Coli and cyanobacteria 5. In the cloning of viral RNA, NaOH is used to remove the RNA template with poly- A tails as RNAs make 2’ to 5’ phosphate linkage under high pH Select the best answer: A. None of the above B. 1, 2, 3 C. 3, 5 D. 4 E. 5 20. Which statement(s) is/are true about divided genome viruses? 1. When only the 3 largest RNAs of Brome Mosaic Virus (BMV) are added to host
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Unformatted text preview: plant cells, the plant shows disease symptoms but no viral particles are made because the dicistronic RNA3 of TMV cannot code for functional capsid proteins 2. The RNA4 of BMV is a subgenome of the RNA3, and replicates through the formation of replicative form (RF) to produce functional capsid protein 3. When the German strain of Tobacco Rattle Virus (TRV) short particles and the American strain of TRV long particles are added to the same host cell, all the TRV RNAs become coated with the capsid protein of the American strain 4. When only the 3 largest particles of Alfalfa Mosaic Virus (AMV) are added to host plant cells, AMV is not infectious because polymerases cannot be translated without RNA4 5. In AMV, although coat gene is on RNA3, coat protein is not produced as the gene is not translatable Select the best answer: A. None of the above B. 1, 2, 3 C. 3 D. 4, 5 E. 5 © 2006. A+PREP, Inc....
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