1. Plaque Forming Unit (P.F.U.) is the amount of virus necessary to produce one plaque 2. In complement fixation test, the amount of non-fixed complement can be determined by the lysis of sheep red blood cells as viruses use complement to lyse sheep red blood cells 3. Pre-natal infection of Rubella Virus can be recognized by immuno-diffusion assay as antibodies raised against Rubella Virus in affected infants will react with the antigen and form a white precipitate 4. In ELISA assay, the presence of antigen-antibody conjugate can be determined by back calculation 5. In neutralization test, only neutralizing antibodies can neutralize viral infectivity Select the best answer: A. None of the above B. All of the above C. 1, 5 D. 2, 3, 4 E. 3, 5 35. Which statement(s) is/are true about stages of viral replication? 1. In Tobacco Mosaic Virus (TMV), replicases bind to the internal promoters on the positive mRNA strand and make subgenomic mRNAs, in order to amplify the expression of capsid proteins
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