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3K-108 - Net ID_KEY A BIOBM3310 Quiz 3a 1(12 pts Consider...

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Net ID ___KEY A BIOBM3310 Quiz 3a 9/24/08 1. (12 pts) Consider the experiment of the steps of unfolding, then re-folding myoglobin, Mb. Draw the indicated graphs. N = native structure a. the holoprotein under physiological conditions b. the holoprotein, but at pH 3.5 and 8M urea c. after step (b), then dialyzing away the urea, but pH still 3.5 d. after step (c), then raising pH to 7 (and adding back heme) 2. (5 pts) Consider the general nature of the process, UNFOLDED PROTEIN FOLDED PROTEIN, as the protein comes off the ribosome. Answer - if negative or + if positive for each of the following questions: a. G o for the folding process _ - _ b. H o for the folding process _ - __ c. T S o for the folding process _ - __ d. T S o for the protein only (not including the water) _ - __ e. T S o for the water only (not including the protein) _ + __ 3. (8 pts) Calculate the magnitude of the free energy change by which prolyl isomerase lowers the barrier for cis trans interchange, given that: (RATE cat / RATE uncat ) = (exp - G o ± /RT ) cat / (exp - G o ± /RT ) uncat R = 0.00832kJ/mol x K and T = 300K RATE cat /RATE uncat = 3000. Circle your answer! Use correct units. 3000 = e - ( Go/RT) ln(3000) = - ( Go/RT) 8.0 = - ( Go)/2.5kJ/mol ( Go) = 20kJ/mol 4. (5 pts) These questions refer to the PyMOL assignment and the projected image on the screen. Images A, B, C all show Pyruvate Kinase.
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