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Unformatted text preview: Net ID__KEY A _________ QUIZ 4a 10/1/08 1. (11 pts) Draw O 2 binding graphs . LABEL BOTH AXES . Show oxygen binding , not the binding of any other molecules. Write THREE numerical values on each axis, e.g. "0, 100, 200" or whatever . Show units, if any, on axes. Label curves as A, B, C, D, E A . n = 3, P50 = 50 mmHg B . n = 1.5, P50 = 2 mmHg C . n = 2.8, P50 = 25 mmHg D . n = 2.8, P50 = 20 mmHg E . n = 1, P50 = 100 mmHg 2. (9 pts) Write R if the relaxed state is favored by the procedure below , or T if the tense state is favored, or N if there is a negligible effect of the following procedures: R pH is raised from 6.6 to 7.1 N O 2 concentration in the lungs is raised from pO 2 = 100 mmHg to pO 2 = 120 mmHg R [BPG] in red blood cells drops in half T CO 2 concentration in red blood cells increases sharply T α 2 γ 2 fetal Hb becomes α 2 β 2 adult Hb T tissue near the red blood cells becomes metabolically active R O 2 concentration is raised from pO 2 = 10 mmHg to pO 2 = 90 mmHg R Hb is mutated from normal adult Hb to a Hb with Hill Equation parameters n = 2.8, P 50 = 2 mmHg R _ The two His143 binding to BPG are mutated to Ser 3. (4 pts) The equilibrium constant K eq for the binding of O 2 to Mb is 1 x 10 6 M-1 , where K eq = [Mb O 2 ]/[Mb][O 2 ]. The rate constant for combination of O 2 with Mb is 1 x 10 7 M-1 sec-1 . What is the rate constant , including units, for dissociation of O 2 from Mb? K eq = k assoc /k dissoc = 1 x 10 6 M-1 = (1 x 10 7 M-1 sec-1 )/k dissoc k dissoc = (1 x 10 7 M-1 sec-1 )/(1 x 10 6 M-1 ) = 10 sec-1 4. (6 pts) Refer to the color projected image from the PyMOL Hb exercise. Write 4....
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- Fall '07
- Hemoglobin, red blood cells, BPG