7K - Net ID KEY A BioBM3310 QUIZ 7a 11/4/09 fumarate + NADH...

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Net ID KEY A BioBM3310 QUIZ 7 a 11/4/09 1. (9 pts) Given this rxn inside a cell at 310K: fumarate + NADH + H + succinate + NAD + The intracellular conc are: [fumarate] = 0.02mM, [succinate] = 0.01mM, [NADH] = 0.04mM, [NAD + ] = 0.40mM, pH = 7.0 The half rxns are: fumarate + 2H + +2e - succinate E o ' = +0.030V R = 8.3x10 -3 kJ/mol K NAD + + H + + 2e - NADH E o ' = -0.30V F = 96.5kJ/V mol a. calculate E o ' +0.30V + 0.03V = +0.33V b. calculate G o ' G o' = -nF E o' = -2(96.5kJ/V mol)(0.33V) = -64kJ/mol c. calculate G G = G o' + RTln[succinate][NAD+]/[fumarate][NADH] = -64kJ/mol + (2.57kJ/mol)ln(.01)(.40)/(.02)(.04) = -64kJ/mol + (2.57kJ/mol)ln5 = -64kJ/mol + 4kJ/mol = -60kJ/mol 2. (2 pts) Write the overall reaction of glycolysis. No need to show chemical formula or structures of reactants and products. Glucose + 2NAD+ + 2ADP + 2Pi 2Pyruvate + 2NADH + 2ATP + 2H2O + 2H+ 3. (13 pts) Rxns of glycolysis are shown below, numbered 1 - 10. Glycolytic intermediate molecules are lettered A - L. Answers can be used more than once, and some blanks require more than one answer . Draw in the missing 3-carbon molecule I. a. 1, 3, 10 Which numbered step(s) have enzymes that are not fully active? d. H, K Which lettered intermediate(s) is a so-called “high energy” molecule? b. ___ 6 ___ Which numbered step(s) are redox rxns? e. 7, 10 _ Which step(s) has ATP as a product? c. 2, 5, 8 Which numbered step(s) are isomerization rxns? 4. (6 pts). Circle T if true, F if false, about the following statements: a. T / F For the majority of the 10 steps of glycolyis, the enzyme is present and highly active. b. T / F
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This note was uploaded on 09/19/2010 for the course BIOBM 3310 taught by Professor Feigenson,gw during the Fall '07 term at Cornell University (Engineering School).

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7K - Net ID KEY A BioBM3310 QUIZ 7a 11/4/09 fumarate + NADH...

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