MUTK'09 - Net ID 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17...

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Net ID KEY BIO3310 MAKE-UP MIDTERM 10/24/09 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. TOTAL BE SURE TO WRITE/PRINT LEGIBLY. IF WE CAN'T READ IT, WE CAN'T GRADE IT! SERIOUSLY. PRINT NAME (LAST, FIRST) ON THE BACK TOP OF THE LAST PAGE ONLY Use these pK a values unless others are given in a problem: terminal COOH 4 terminal NH 3 + 9 R-GROUPS: Asp 4 Glu 4 Cys 8 Tyr 10 His 6.5 Lys 10
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1. (6 pts) In the T state of Hb, one possible ion pair is between His 143 and BPG. Circle the fraction of His-BPG that could actually be in an ion pair at pH values shown, given His 143 pK a = 6.5. Assume BPG is fully ionized. pH = 5.5 pH = 6.5 pH = 7.5 1.0 1.0 1.0 0.0 0.0 0.0 0.5 0.5 0.5 0.1 0.1 0.1 0.9 0.9 0.9 2. (5 pts) Consider two proteins, A and B. Protein A has a single subunit. Protein B is a dimer, represented as x 1 y 1 . The polypeptides A, x, and y all have the same tertiary structure, but are all about 75% different in AA sequence. Circle T if true, F if false, for the following statements. T / F A, x, and y have the same IV structure. T / F A, x, and y have the same protein fold, also known as the structural domain. T / F Protein B might be regulated by an R - T transition of its IV structure, but protein A cannot be regulated this way. T / F Ramachandran Maps of A, of x, and of y must look nearly identical. T / F Even if the tertiary structure of A, x, and y is the same, the II might well be quite different. 3. (5 pts) An enzyme requires a protonated Lys (pK a 10) and an unprotonated His (pK a 6) for catalytic activity. Draw the expected graph of V max vs pH. Label the axes showing numerical values of pH. 4. (3 pts) Define holoprotein (
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This note was uploaded on 09/19/2010 for the course BIOBM 3310 taught by Professor Feigenson,gw during the Fall '07 term at Cornell.

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MUTK'09 - Net ID 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17...

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