bio lab 2 - Determination of the Acid Dissociation...

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Determination of the Acid Dissociation Constants of an Amino Acid Introduction: The purpose of this lab is to determine the acid dissociation constants of each of the ionizable groups of glycine by performing titrations using 0.2 M NaOH. The titration curves will probably provide evidence of the buffering effect occurring, where a large amount of added NaOH results in a small pH change and almost a vertical line on the curve. The hydroxide ions added are neutralized by hydrogen ions released by an ionizable group. Glycine will most likely have the greater acid dissociation constant (lower pKa value) because it has two ionizable groups as opposed to the one of acetic acid so it can form dipolar ions. Acetic acid will have a larger pKa value for its carboxyl group than glycine’s because it is a weak acid. Glycine will have two pKa values since it is an amino acid (one for amino group and one for carboxyl group). Procedure:
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bio lab 2 - Determination of the Acid Dissociation...

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