bio lab 4 - Measure of Light Intensity and Wavelength...

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Measure of Light Intensity and Wavelength Absorption Through the Separation of Pigments by Chromatography Abstract: The objective of this lab was to separate the pigments chlorophyll a, chlorophyll b, and carotenoid from a piece of spinach by the process called chromatography. The three pigments were isolated and then extracted from the spinach with the lipid solvent acetone. A spectrophotometer was used to determine the absorption spectra of each of the pigments from wavelength 400-700. Our results showed that the rate of photosynthesis in plant cells is dependent on the light it receives. The lower the wavelength was, the higher the absorbance. Also, lighter pigments moved faster than darker ones because they were more soluble. Since the principal pigment of chloroplasts is chlorophyll a, it had the greatest measures of absorbance out of the three pigments. Chlorophyll b and yellow carotenoids play secondary roles in chloroplasts, so their absorbances were noticeably smaller. Under UV light, carotenoids protect photosynthetic systems from damaging effects and were fluorescent. The chlorophylls were also
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This note was uploaded on 09/20/2010 for the course BIOLOGY 2050 taught by Professor Coombs during the Spring '10 term at Adelphi.

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bio lab 4 - Measure of Light Intensity and Wavelength...

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