bio lab 5 - Analysis of Spliced DNA Through Gel...

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Analysis of Spliced DNA Through Gel Electrophoresis Abstract: The purpose of this experiment was to determine the size of already cut pieces of DNA using the process of gel electrophoresis, which separated the DNA fragments according to size. We found that PstI restriction enzyme produced the most lambda DNA fragments, so it had some smaller molecules than the other enzymes. The uncut lambda DNA only produced one fragment because it was so large and the HindIII produced more fragments than the EcoRI. Procedure: Obtain four microtubes and label them as L (uncut lambda DNA), P (Pstl lambda digest), E (EcoRI lambda digest), and H (HindIII lambda digest). Using a new pipet each time, transfer 10 ul of the appropriate DNA sample to each test tube. Add 2 ul of loading dye to each tube and mix the contents either by centrifuging or flicking the tube lightly. For the gel electrophoresis, prepare an agarose gel with wells and place it in the electrophoresis chamber. Fill the chamber and cover the gel with buffer. Make sure
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This note was uploaded on 09/20/2010 for the course BIOLOGY 2050 taught by Professor Coombs during the Spring '10 term at Adelphi.

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