AP Bio dna restriction lab - Restriction Digestion and...

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Restriction Digestion and Analysis of Lambda DNA Lab Doyun Kim, Hannah Kim , Jae Woong Kim, Crystal Yun January 30, 2010 AP Biology Period 10
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Abstract After conducting this lab, we will understand how restriction enzymes help biotechnology, explore how gel electrophoresis works, and learn to generate a standard curve from the resulted DNA size fragments, and be able to estimate DNA fragment sizes from the data. First, we will cut DNA from lambda, a bacterium-invading virus. Then, the DNA molecules are separated and finally their sizes are determined by gel electrophoresis. Introduction The objective of this lab is to determine the size of the DNA molecules by gel electrophoresis. This procedure is useful for a variety of situations ranging from DNA fingerprinting to gene localization. Scientists cut and link DNA molecules in biotechnology in order to study how certain genes work. In this lab, lambda DNA is cut into a series of fragments using three different restriction enzymes, also called endonucleases (PstL, EcoRI, Hindlll). Each enzyme cuts
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This note was uploaded on 09/22/2010 for the course ABD. 39283 taught by Professor Dr.met during the Spring '10 term at Aachen University of Applied Sciences.

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AP Bio dna restriction lab - Restriction Digestion and...

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