DNA webquest answers - HannahKimPeriod10 Mr.Paulson...

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Hannah Kim Period 10 Mr. Paulson  DNA extraction Why would we need to extract DNA from human cells? Describe each of the four steps in the process of isolating DNA 1) collect cheek cells 2) burst cells open to release DNA by using the lysis solution to burst the cells.  Must stay in warm water bath  3) separate DNA from proteins and debris -add salt 4) isolate concentrated DNA -put in centrifuge to separate the debris from DNA What are the components and functions of Lysis solution? The lysis solution contains detergent and proteinase K. The detergent disrupts the cell membrane and  nuclear envelope so that the cells can burst and release their DNA. The proteinase K then cuts the histones apart  to free the DNA. Why is the concentrated salt solution necessary? The salt causes proteins and other cellular debris to clump together, causing the DNA to separate from  proteins and debris. What does adding isopropyl alcohol do and why? Since DNA is not soluble in isopropyl alcohol, it comes out of the solution.
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This note was uploaded on 09/22/2010 for the course ABD. 39283 taught by Professor Dr.met during the Spring '10 term at Aachen University of Applied Sciences.

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DNA webquest answers - HannahKimPeriod10 Mr.Paulson...

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