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Unformatted text preview: [Xq “ * ] [8q  “ * ] Determining the Sucrose Concentration Using Osmosis Placing six dialysis bags filled with water in the six unknown concentrations of sucrose, we found the percent change in mass of each bag and identified the concentrations of the unknown solutions. Hannah Kim Mr. Paulson per 10 October 1, 2009 1 [H q “ * ] [x p ] “ * ] Introduction The purpose of this experiment is to determine the concentration of sucrose of six unknown concentrations. The six concentrations are 0M, 0.2M, 0.4M, 0.6M, 0.8M, and 1.0M. However, we do not know which beaker contains which concentration. Therefore, we need to find out the concentrations of sucrose using osmosis. Osmosis is the diffusion of water through a membrane that is selectively permeable. Osmosis is especially important in living organisms because all cells need the process of osmosis to balance out the concentrations of solute. Water moves from an area of high water potential to an area of low water potential. The measurement of free energy of water in a solution is the water potential. If the solute concentration is higher inside the membrane than the outside, then the cell is hypertonic; if the solute concentration is lower on the inside and higher on the outside, then the cell is hypotonic. Hence, water will move from higher concentration to the area with lower concentration to balance the concentrations out. We decided to use six dialysis bags to observe the osmosis movement in each of the six beakers of different concentrations. I predict that the dialysis bag that has no change in mass has zero concentration of sucrose. This is an isotonic environment, where there is no net change in mass because both the beaker and the dialysis bag do not contain sucrose. Also, the dialysis bag that lost the most water to the beaker will have the highest concentration of sucrose (1M) because since the concentration of sucrose is...
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This note was uploaded on 09/22/2010 for the course ABD. 39283 taught by Professor Dr.met during the Spring '10 term at Aachen University of Applied Sciences.
- Spring '10