Separation_fish_proteins - Gel Electrophoresis of Proteins...

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Gel Electrophoresis of Proteins Comparative Proteomics: Protein Profiles of Fish Muscle Tissue adapted by Audrey Dell Hammerich 10/2009 Electrophoresis is an analytical method in which substances are separated from one another based upon their differing rates of migration in a strong electric field. In this lab you will use protein gel electrophoresis, the technique most widely used in biotechnology research, to examine muscle proteins from closely and distantly related fish species, and to identify similarities and differences in these organisms’ protein profiles (fingerprints). Muscle protein consists mainly of actin and myosin, but numerous other proteins also make up muscle tissue. While actin and myosin are highly conserved across all animal species, the other proteins are more diverse, varying even among closely related species. Proteomics and Evolution Proteomics is the study of proteins, particularly their structures and functions. This term was coined to make an analogy with genomics, and while it is often viewed as the “next step”,
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This note was uploaded on 09/23/2010 for the course CHEM 222 taught by Professor Staff during the Spring '08 term at Ill. Chicago.

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Separation_fish_proteins - Gel Electrophoresis of Proteins...

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