TheKNOWListSp2010 - BIS103 Spring 10 FINAL EXAM Info for...

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Unformatted text preview: BIS103 Spring 10 FINAL EXAM Info for Monday June 7 10:30-12:30 pm Bring a calculator (there may be some calculation quest io ns) Use the chat for questions! I will answ er as fast as I can a nd others can answ er as w ell! Exam will be part over the mater ial after the second mid ter m (about 40-50%) and the r est cu mulative. Material af ter seco nd midterm: Ligh t reactions of PS, Calvin cycle, aa breakdown in animals, amino transf erase r eactions, aa biosyn thesis, 1 carbon metabolism, N and S assimilation, misc. reaction of aa, intro duction to purin e/pyrimid ines, metabolic in tegration. Structures, be able to recognize AND draw fro m na me: all gly coly tic intermed iates/all TCA cy cle intermed iates/g lyoxylate cycle intermediates other carbohydrates listed in handbook, glycerol amino acids: Cys, Ser , Asp , Asn , G lu, G ln, Ala, Met, Gly , Ph e, Tyr, homoserin e, ho mocysteine, saturated and unsaturated FA, tr iacylg lycerides, malonyl Co A Structures, be able to recognize, not draw--all co-factors d iscussed in class/all electron carr iers (what th ey carry)/photo synthetic pigmen ts, nitrate, nitrite, ammon ia, su lfate, nucleo tides, pur ine, pyrimid ine Pathways know the steps of and structures of a ll the intermediates anaerobic g lycolysis/aerobic gly colysis/catabo lism of g lycerol TCA (Krebs) cycle/glu coneogenesis/glycog en (starch) synth esis/gly cogen (star ch) breakdown/βox. of sat’d F/ aa breakdown: Ala, G ly, Ser, Glu , G ln, A sp, A sn/ aa syn: Glu , G ln, A sp, A sn, Met (not stru ctures of all intermediates), Ser, Cys (not stru ctures of all inter med iates) , G ly, Ala, Tyr (from Ph e only) Other things to know Gibbs standard fr ee energy change vs G ibbs free energy/equilibriu m/K eq/Q /Know how to use ΔG, ΔG0’, ΔE0’ Pathw ays that utilize GLC/Pathways that u tilize acetyl- CoA Vitamin-Co-en zyme relationsh ips/g eneral ro le of each co-factor in enzy me r eaction/g ive example Distinguish between substr ate lev el phosphorylation/ ox idative phosphorylation/ pho tophosphorylation Know reactions of complexes of resp iratory electron transpo rt/reaction of ATP synth ase Overall goal of Calv in cycle/RU BISCO reaction/r eduction p hase (not the regen eration phase) Compar e mito and chloro. ETC/ compar e A TP synthesis/ compare PPP and Calvin cycle Nitrogen /sulf ate r eduction and assimilation/p athw ay for net N assimilation after redu ction to NH3 (which are g lutamate synthase/glu tamin e syn thetase) Know direction of N assimilation- nitrate to nitrite to ammonia to G ln to G lu in those organ isms th at reduce N Know direction of S assimilation- su lfate to sulfid e to Cys to Met in those organ isms th at reduce S Aminotr ansferase reactions/alphaKG as un iversal amino acceptor, Glu as un iversal donor/PLP-dep reactions, especially amino acid amino transfer ases, decarboxylases/THF reactions/Glu dehydrogenase r eaction (GDH) Waste N products in differ ent organisms- NH3 in aquatics, u ric acid in birds, and urea in terrestrial an imals Know net yields of ATP and NA DH and FADH2 for g lycoly sis, TCA cycle, ß-oxid ation Know consumption of A TP for g luconeogen esis, FA synth esis, Calv in cycle Understand concept of shuttling between co mpar tmen ts - shu ttle for moving electrons to mitochondria Subcellular locations in eukaryo tes for glyco lysis, g luconeog enesis, TCA cycle, FA synth esis, ß-oxid ation, sub cellu lar locations in euk for photosynthesis (light r eactions and Calvin cycle) Names of compar tments of mito chondria and ch loroplasts an d what reaction and pathways happen wh ere Fates of acetyl coA in an imals Vs plants- no net CHO syn thesis from FA in animals, but g lyoxylate cycle allows net synth esis of CHO from FA in plants, fungi. Differen ces in protein, amino acid metabolism, N and S assimilation b etw een p lan ts and an imals 1 carbon carriers/d iffer ences and similar ities/methyl cycle- role of 1 carbon carr iers Role of methyl cycle in d TMP synthesis Know these enzyme n ames: kinase, phosphatase, k eto liso merase, ep imerase, decarboxylase, aminotr ansfer ase, oxygenase, dehydrogenase, iso mer ases, hydrolases, transf erases, oxidoreductases, dehydrogenases Activation of groups-add good leaving grp (th ioester formation, acy l phosphate, acetylation, phosphorylation, etc.) Idea and examples of reciprocal r egulation/what is futile cy cling and why fu tile cy cling is useful Kinds of enzyme regu lation-ch anging specific activity ( allosteric regulation, covalen t modification) Enzyme regu lation (Why ? How ? Which ?)/Proper ties of physiologically rever sib le enzy mes Properties of physiologically irr eversible en zymes/Which regulated and why? Common alloster ic regulators, why these? Wh at does F2,6bisP, cA MP do ? Mammalian in tegration of carbohydrate/lipid /protein breakdown by hormones/catabolic/anabolic/amphibolic Summary Sheets are good study aids- Should understand all concepts in summary points EXCEPT: 1. 18.5- remember, newer lactate hypothesis that is not yet in textbooks. 2. NOT 20.6 We did not go over P/O ratios 3. NOT 21.8 We did not go over this reaction. 4. NOT 23.5 We did not go over other ways to oxidize FAs. 5. NOT 24.3 through 24.7 Lots more in this chapter than we covered in class 1 Common Enzymes Names for the Final: Phosphofructokinase (PFK-1) Glycogen (starch) Phosphorylase (or phosphorylase is OK) Debranching enzyme Sucrase Lactase Maltase PDH complex (or pyruvate dehydrogenase) Pyruvate carboxylase Glycogen synthase Starch synthase Branching enzyme Protein kinase A Adenylate cyclase phosphorylase a phosphorylase b Acetyl CoA Carboxylase Isocitrate lyase Malate synthase Complex I Complex II Complex III Complex IV ATP synthase New for final: RuBISCO Alanine aminotransferase Aspartate aminotransferase Glutamine synthetase Asparagine synthetase Glutamate synthase Serine hydroxymethyl transferase Glycine synthase Phenylalanine -4-hydroxylase 2 ...
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This note was uploaded on 09/23/2010 for the course NPB 8746546 taught by Professor Goldberg during the Spring '10 term at UC Davis.

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