Exam_2Answers - l. A. Taking into account the Wobble Rules...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: l. A. Taking into account the Wobble Rules and the Genetic Code Table, what is the minimal number oF distinct tRNA genes required to recognize all Leucine codons? (10 pts.) (Your answer needs to be a single number — circle it.) matador]; ( 5 lykfl 3 S'CM > S 5’MG' 5’5"“, dye. 9'e’w> l ' U I B. What are the anticodon sequences of the tRNAs that these genes encode? (10 pts.) (You must indicate the 5’ erit For your answer.) / 2. A tRNA with the anticodon sequence 5'UAU cannot exist. Why not? (15 pts.) (One sentence answer - if you write more than one sentence — I’m reading only the First one.) _ SIAM-A + 5 (241461 WWWWM mm Mag 7:: was regpwhW-fj , 3. A mutant bacterium has a nonsense suppressor tRNA that inserts glutamine (Gln) to match a 5'UAG codon, but not other nonsense codons. The nonsense suppressor mutation is a single nucleotide change From wild—type. A. What is the anticodon oF the mutant suppressor tRNA? (10 pts.) (Indicate the 5' end and circle your answer iF you want credit.) B. What is the anticodon of the wild-type tRNAG'" that was mutated to generate the nonsense suppressor? (5 pts.) (Indicate the 5' end and circle your answer iF you want credit.) 4 4-. Eight difFerenf mul‘anf sfrains oF mice (A-H) have been isolafed, each showing a recessive curly-fail phenofype. (Normal mice have s’rraighl' fails.) All pairwise crosses between l'he s’rrains were performed, and lhe resulls are shown below. (+) indica’res ‘rhe progeny were wild—lype, and (0) indicates l'he progeny had curly fails. ABCDEFG H AOO+++++ + B O+++++ + C O +@+@+ D O+++ + E O +@+ F 0+ + G O + H O as A. Sori all of fhe s’rrains info complemenl’a’rion groups. (10 pfs.) B. How many different genes are mul'am‘ among These eigh’r s‘rrains oF mice? (5 pls.) (Your answer should be a single number - please circle i’r.) \ 5. The particular SSR locus shown below (x=number oF repeats) is linked to a dominant autosomal disease gene allele, D. A pedigree diagram for a Family with the disease is also shown below. PCR primer 1 —> (AC) x PCR primer 2 PCR products using primer1 and primer 2 A. Indicate the most likely configuration oF the normal (d) and mutant (D) alleles of the disease gene with respect to the particular SSR alleles in each of the two parents. (10 pts.) D 30 $ (15 SO ____._ /___d cl so (no B. Circle anyone in the diagram who appears to be a recombinant. (10 pts.) C. Using only the data in this diagram, what is the recombination Frequency between the SSR locus and gene D? (5 pts.) Rial/g 6. Below are 5 DNA Fingerprints. The probe is complementary to the repeat sequence of a particular VNTR. Mrs Mr. Ronald James Susan Larson Larson Scott Scott Scott l______—M — — — - ' — _ 1 _ - — — l — — — _ - — — I _ = — I - - — - = - - l — _ _ — — _— _ - - A. Mrs. Larson is Ronald Scott's biological mother. Who of the above is his biological Father? (4 pts.) Mr. l ay‘SOfl B. How many bands would there be in each lane of the autoradiograph if a probe to unigue sequence adjacent to one of the VNTR loci were used rather than a probe corresponding to the VNTR repeat itself? (6 pts.) mbalol 2. TlEcassiblvlp‘ i— lOU/fi ES WOZVSW BONUS QUESTION The human pedigree diagram shown below shows people afFec’red wi’rh a rare disease called nail-pafella syndrome (misshapen nails and kneecaps) and also gives the ABO blood group genotype oF each individual. Bo’rh loci (NP and I) are aul'osomal and they are linked. A. Circle the individuals in 1'he diagram who are recombinanfs. B. Considering The da’ra in this pedigree only, whal is the RF be’rween the NP and I genes? (circle your answer) ...
View Full Document

Page1 / 6

Exam_2Answers - l. A. Taking into account the Wobble Rules...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online