Exam4TTKey - BIOL 3301 EXAM 4 Section 07870 GENETICS...

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BIOL 3301 Section 07870 GENETICS EXAM 4 Thursday April 24, 2003, 5.30-7.00 pm NOTE: MAKE SURE YOU READ THE QUESTIONS CAREFULLY!!! ________________________________________________________________________ Name: SSN: ________________________________________________________________________ True-false statements (write True or False in front of the statement) (2 points each, total 20 points). 1. Dideoxy-sequencing was devised by Maxam and Gilbert. F 2. The blue-white screen for recombinant plasmids involves the tetracyclin-resistance gene. F 3. Southern blotting is used for the analysis of total RNA. F 4. DNA fingerprinting in forensic science and in paternity tests makes use of VNTRs. T 5. SNPs enable the most refined mapping of genes on chromosomes. T 6. A forward mutation is any change back to the wildtype allele. F 7. Alkyltransferase is required for direct reversal of photodimers. F 8. Intercalating agents lead to frameshift mutations. T 9. Cyclins act to control the progression of the cell cycle by dephosphorylating Rb protein. F 10. The normal activity of the RB protein is to negatively regulate the progression from G1 to S of the cell cycle. T
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Multiple choice (circle the correct answer) (2 points each, total 50 points). 1. The recognition sequence for the restriction site EcoRI is: a. GGATCC b. GAATTC c. GGGCCC d. AGATCT e. TCTAGA 2. Complete the following statement by picking the correct pair of words from the list. 'A critical feature of cloning plasmids is the presence of a ……. ................. marker such as . ................. ……………. resistance'. - visible & blue-white - selectable & ampicillin - lethal & tetracyclin - none of the above 3. The blue-white screen for recombinant plasmids depends on: a. alpha complementation of β -galactosidase b. antibiotic resistance c. permease activity d. replica plating e. luck 4. A plasmid vector is cut in one place and a poly(G)tail is added to the 3’ ends. For effective cloning, the donor DNA should have a. poly(G) at the 3’ ends b. poly(G) at the 5’ ends c. poly(C) at the 3’ ends d. poly(C) at the5’ ends e. poly(G) at the 5’ ends and poly(C) at the 3’ends 5. First strand cDNA is synthesized from mRNA by means of: a. DNA polymerase I b. DNA ligase c. S1 nuclease d. Reverse transcriptase e. RNA polymerase III 6. In the dideoxy sequencing method the use of dideoxy thymidine triphosphate stops
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This note was uploaded on 09/25/2010 for the course BIOL 3301 taught by Professor Gunaratne during the Fall '05 term at University of Houston.

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Exam4TTKey - BIOL 3301 EXAM 4 Section 07870 GENETICS...

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