GenCh8answers - 2. In prokaryotes, translation is beginning...

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2. In prokaryotes, translation is beginning at the 5´ end while the 3´ end is still being transcribed. In eukaryotes, processing (capping, splicing) is occurring at the 5´ end while the 3´ end is still being transcribed. 4. Sigma factor, as part of the RNA polyermase holoenzyme, recognizes and binds to the –35 and –10 regions of bacterial promoters. It positions the holoenzyme to correctly initiate transcription at the start site. In eukaryotes, TBP (TATA binding protein) and other GTFs (general transcription factors) have an analagous function. 5. The CTD (carboxy tail domain) of the ß subunit of RNA polymerase II contains binding sites for enzymes and other proteins that are required for RNA processing and is located near the site where nascent RNA emerges. If mutations in this subunit prevent the correct binding and/or localization of the proteins necessary for capping, then this modification will not occur even though all the required enzymes are normal. 9.
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GenCh8answers - 2. In prokaryotes, translation is beginning...

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